Skip to main content
. 2009 Apr 3;5(4):e1000443. doi: 10.1371/journal.pgen.1000443

Figure 5. Gene dosage effect of Hes1 on taste cell differentiation.

Figure 5

(A) CVP from E18 embryos of wild-type and Hes1 −/− mutant littermates were stained with antibodies against PLCβ2 and IP3R3. The developing taste buds from the oral epithelium of Hes1 −/− mutants (lower panels) exhibited many more PLCβ2 (green) and/or IP3R3 (red) immunoreactive cells than did their wild-type littermates (upper panels), which displayed very few PLCβ2 and IP3R3 immunoreactive cells at this stage. Scale bar, 10 µm. (B) PLCβ2 and IP3R3 immunoreactive cells in Hes1 mutant CVP at E18 and P0. Serial sections of entire CVP from wild-type, Hes1 +/−, and Hes1 −/− littermates were immunostained with the PLCβ2 and IP3R3 antibodies, and immunoreactive cells were counted. The results represent the mean of more than five specimens. (C) The table shows the average and standard deviation (S.D.) of PLCβ2 and IP3R3 immunoreactive cells at E18 and P0 obtained from sections of entire CVP from wild-type, Hes1 +/−, and Hes1 −/− littermates. The table is graphically displayed in Figure 5B. More than five specimens of each genotype and stage were used for counting the cells.