Figure 4.

Correlation between methylation of ATM mvp2b and expression of ATM in cancer cell lines. (A) Methylation analysis in 62 cancer cell lines reveals a similar range of methylation as in PBMCs of breast cancer patients (77–99%); however, the distribution is skewed towards increased methylation in the cancer cell lines (green). Methylation distributions of breast cancer patients (red) and controls (black) are shown for comparison. (B) ATM expression was determined by qRT–PCR in the five breast cancer cell lines and by gene expression microarray data (C) for a panel of sarcoma cell lines. Error bars represent SEM from triplicate qRT–PCR experiments. Correlation between methylation and expression is shown using Spearmans rank correlation coefficient.