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. 2009 Jan 7;18(7):1181–1189. doi: 10.1093/hmg/ddp009

Figure 1.

Figure 1.

Subnuclear localization of CRM1, PHAX, C/D box snoRNP component fibrillarin, H/ACA box snoRNP proteins GAR1 and NAP57/dyskerin, snoRNP chaperone Nopp140 and coilin in SMA-derived fibroblast cells. Immortalized control and Type I SMA patient fibroblasts were costained for CRM1 (A), PHAX (B), fibrillarin (C), GAR 1 (D), NAP57/dyskerin (E) or Nopp140 (F) in green and the CB marker coilin in red. CRM1 and PHAX nuclear foci in both control and SMA cells were localized in CBs, as shown in yellow (merge). The arrow points to one of the CBs. It should be noted for both control and SMA cells that most of the immunolabelling for fibrillarin, GAR1, NAP57/dyskerin or Nopp140 concentrates in nucleoli (arrowhead) as expected. Fibrillarin nuclear foci (arrow) in both control and SMA cells were localized with coilin in CBs, as shown in yellow. GAR1, NAP57/dyskerin and Nopp140 nuclear foci (arrow) were found colocalized with coilin in control cells. In contrast, only a few Nopp140 foci (arrow) were detected in SMA cells and were not colocalized with coilin. The arrowhead and arrow point to one of the nucleoli and one of the CBs, respectively. Scale bars, 3 µm.