Figure 5.

Comparison of the localization of Nopp140 in CBs of cells transfected with wild-type SMN and SMA mutants. The SMN protein is depicted with the Lys-rich, Tudor, Pro-rich, oligomerization YG-box and ex7 encoded domains. SMN and SMA mutants were fused to the N-terminal end of eGFP (A, 36). The constructs indicated were transiently transfected into COS cells (B). Overexpression of eGFP-SMN (green) enhances the localization of Nopp140 to CBs (red), as observed in yellow (merge). The arrow points to one of the CBs. While the SMA mutants SMNE134K, Δex7 and 472Δ5 do not. The data are quantified in table (C). Scale bars, 3 µm.