Figure 6.

Subnuclear localization of Nopp140 in primary fibroblasts derived from control and Type I, II and III SMA patients. Primary fibroblasts were immunostained for two CB markers SMN (A) or coilin (B) in green and Nopp140 in red. Colocalization in CBs (arrow) is shown in yellow (merge). The arrowhead and arrow point to one of the nucleoli and one of the CBs, respectively. The statistical analyses show a correlation between Nopp140-positive CBs and SMA disease severity and are presented in a histogram (C) and in Table 2. Scale bars, 3 µm.