Table 1.
Analyses of nuclear foci in immortalized Type I SMA-derived fibroblast cells
| Immortalized fibroblasts | Proportion of cells witha |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SMN foci (%) | CBs with SMN (%) | CRM1 foci (%) | CBs with CRM1 (%) | PHAX foci (%) | CBs with PHAX (%) | Fib foci (%) | CBs with Fib (%) | CBs with GAR1 (%) | CBs with NAP57 (%) | Nopp140 foci (%) | CBs with Nopp140 (%) | |
| Control | 59 (n = 600) | 64 (n = 200) | 11 (n = 200) | 11 (n = 200) | 21 (n = 200) | 21 (n = 200) | 43 (n = 300) | 37 (n = 60b) | 23 (n = 505) | 36 (n = 438) | 44 (n = 200) | 41 (n = 200) |
| SMAI | 6 (n = 870) | 6 (n = 870) | 23 (n = 100) | 23 (n = 100) | 14 (n = 200) | 14 (n = 200) | 16 (n = 386) | 16 (n = 386) | 6 (n = 425) | 5 (n = 559) | 10 (n = 400) | 1 (n = 100) |
n corresponds to the number of cells that were counted.
afoci were identified with antibodies to one protein only. CBs were identified with antibodies to coilin.
bOnly the cells with fibrillarin (Fib) foci were considered for the statistical analyses.