Table 3.
Antibodies used in the IF studies
| Antibody | Host | Fixation | IF dilution | Source |
|---|---|---|---|---|
| Anti-SMN | Mouse | Formaldehyde (FAH) | 1:500 | Transduction Laboratories |
| Anti-coilin | Mouse | FAH | 1:1000 | Transduction Laboratories or Sigma-Aldrich |
| Rabbit | FAH | 1:1000 | Santa Cruz | |
| Anti-Nopp140, RS8 | Rabbit | FAH | 1:5000 | (59) |
| Anti-NAP57 | Rabbit | FAH | 1: 250 | (50) |
| Anti-GAR1 | Rabbit | FAH | 1: 250 | From W. Filipowicz |
| Anti-fibrillarin | Rabbit | FAH and MetOH | 1:1000 | Abcam |
| Anti-PHAX | Mouse | FAH and MetOH | 1:1000 | From I. Mattaj |
| Anti-CRM1 | Rabbit | FAH and MetOH | 1:2000 | Abcam |