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. 2009 Jan 15;18(7):1229–1237. doi: 10.1093/hmg/ddp023

Figure 2.

Figure 2.

Tannic acid alleviates aberrant inclusion of CHRNA1 exon P3A due to IVS3-8G>A by facilitating the expression of PTBP1. (A) RT–PCR of wild-type and mutant CHRNA1 minigenes in HEK293T cells with increasing concentrations of tannic acid. (B) Real-time RT–PCR to quantify the ratio of P3A-skipped transcript arising from wild-type and mutant CHRNA1 minigenes in HEK293T cells with increasing concentrations of tannic acid. The ratios are represented by the mean and SD. For the mutant minigene, 100 µm tannic acid increases the ratio of the P3A(−)-transcript. (C) Real-time RT–PCR to demonstrate a dose-dependent increase of PTBP1 mRNA by tannic acid. The ratios are normalized to that in the absence of tannic acid. Means and SD are represented.