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. 2009 Feb 20;106(10):3806–3811. doi: 10.1073/pnas.0900244106

Fig. 1.

Fig. 1.

LL-37 mediates MSC migration and invasion through a G protein-coupled receptor. (A) FPRL1 expression on 3 different donor pools of MSCs analyzed by flow cytometry. (B) Graphic representation of MSC migration stimulated as indicated in a modified Boyden chamber. EGF and PMA were used at 10 ng/mL. (C) MSC migration after pretreatment of cells with 100 ng/mL pertussis toxin (Ptx), or preincubation of LL-37 and EGF with an anti-LL-37 neutralizing antibody (αLL-37). (D) Invasion of MSCs through Matrigel-coated inserts following stimulation as indicated. (E) MSC invasion after pretreatment of cells with Ptx or preincubation of LL-37 and EGF with αLL-37 antibody. *, P < 0.05; **, P < 0.01. (F) Lysates from LL-37-treated MSCs analyzed for ERK phosphorylation by Western blot. MSCs in the far right lane were pretreated with Ptx for 1 h before stimulation with LL-37 for 10 min. M = molecular weight marker. (G) Quantification of Western blot band intensity by densitometry (n = 3), plotted as a bar graph.