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CTL-mediated islet damage initiates transient OVA presentation. RIP-OVAlo mice were injected i.v. with 0.22 × 106 CTLs and then left for 4, 7, 14, 21, 28, or 42 days before being injected i.v. with 2 × 106 CFSE-labeled OT-I cells. Proliferation was examined by flow cytometry in the draining pancreatic and nondraining inguinal lymph node 60 h after CFSE OT-I injection. Representative data from 3 independent experiments are shown.
