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. 2009 Feb 20;106(10):3901–3906. doi: 10.1073/pnas.0810427106

Fig. 3.

Fig. 3.

CTL-mediated islet damage initiates cross-presentation by DCs. (A) bm1→RIP-OVAlo and control B6→RIP-OVAlo bone marrow chimeras were injected i.v. with 0.22 × 106 CTLs and then 4 days later were injected i.v. with 2 × 106 CFSE-labeled OT-I cells. Proliferation was examined by flow cytometry of the draining pancreatic and nondraining inguinal lymph node cells 60 h after injection of CFSE OT-I cells. Representative data from 3 independent experiments are shown. (B) CD11cDTR→RIP-OVAlo chimeras were given 0.22 × 106 CTLs i.v. and then injected i.p. with either PBS (PBS) or 100 ng diphtheria toxin in PBS on days 1, 3, and 6 postinjection of CTLs. On day 4 after CTL injection the mice were given 2 × 106 CFSE-labeled naive OT-I cells i.v. and their proliferation was examined as in A. Representative data from 2 independent experiments are shown. (C) B6→RIP-OVAlo.bm1 bone marrow chimeras and control RIP-OVAlo.B6 mice were given 107 CTLs i.v. followed by 2 × 106 CFSE-labeled OT-I cells i.v. 4 days later. Proliferation was examined as in A. Representative data from 2 independent experiments are shown.