Fig. 5.

gB antigen released from the skin of K14-gB mice by CTL killing induces naive gBT-I deletion. (A) K14-gB mice were given either 7.5 × 10⁶ in vitro activated gBT-I CTL (DAMAGE) or media (NO DAMAGE) i.v. and then 7 days later were injected i.v. with 2 × 10⁶ CFSE-labeled Ly5.1⁺ naive gBT-I cells. Proliferation was examined by flow cytometry of the pooled skin draining lymph node cells 60 h after injection of CFSE gBT-I cells. Representative data from 7 independent experiments are shown. (B) K14-gB mice were given either media (NO DAMAGE) or 7.5 × 10⁶ Ly5.2⁺ CTLs (DAMAGE) i.v. followed by 2 × 10⁶ Ly5.1⁺ naive gBT-I cells i.v. 4 days later. Six weeks after naive gBT-I injection, the number of Ly5.1⁺ CD8⁺ cells (derived from the original naive Ly5.1⁺ gBT-I cells) remaining in the spleen and lymph nodes of the mice was determined by flow cytometry. Pooled data are shown from 2 independent experiments. ***, P < 0.001. Circles represent individual mice and the bar represents the average.