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Foxy-5 up-regulates ERα in vivo. 4T1 breast cancer cells (2.5 × 104) were inoculated into the mammary fat pads of 8-week old Balb/C mice. The animals were subsequently treated with either PBS alone, the Rdm control peptide (20 μg), or Foxy-5 (20 μg) every 4th day for 25 days. RNA was extracted from primary breast tumors from four animals in each group and subjected to RT-PCR for murine ERα. Shown are primary tumor samples from two animals of each treatment group.
