Fig. 1.

α-Hemolysin–induced hemolysis in equine, murine and human erythrocytes. (A) Effect of α-hemolysin containing E. coli (ARD6, serotype OK:K13:H1) supernatant on human erythrocytes attached to a coverslip after 10, 20, and 60 minutes' incubation at 37 °C (see also Movie S1). (B) Summarized data. The total amount of crenated erythrocytes (open columns) and lysed erythrocytes (dotted columns) over time analyzed in image sequences collected over 60 minutes, at 0.1 Hz (n = 8 human). (C) The overall hemolysis is shown as an increase in optical density at 540 nm (OD540) reflecting the hemoglobin concentration in the solution. The erythrocytes were incubated with E. coli supernatant (50 μl·ml⁻¹) from 0 to 60 minutes. n = 5, 7, and 6 for equine, murine, and human, respectively.