Fig. 3.

HlyA-induced hemolysis is inhibited by P2X₇ receptor antagonists. HlyA-induced hemolysis in human (squares), mouse (filled circles), and horse (open circles). Hemolysis induced by HlyA-producing E. coli was reduced by increasing concentrations of (A) Evans Blue, (B) KN-62, and (C) Brilliant Blue G (BBG). (D) Concentration-dependent effect of BBG at various concentrations of purified HlyA. ATP-2′,3′-dialdehyde (OxATP) (E) likewise reduced the hemolysis induced by HlyA-producing E. coli and by the purified toxin (inset, OxATP, 500 μM). (F) The selective P2X₇ antagonist A438079 showed an effect mainly on human erythrocytes. Values are mean ± SEM, n = 5–13. (G) Immunoblots with a C-terminal antibody directed against P2X₇ receptor (dilution 1:200). Left panel show a similar blot with peptide preadsorption.