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. 2008 Nov 7;10(6):R130. doi: 10.1186/ar2545

Figure 1.

Figure 1

Synthetic peptides number 18 and 25 produced marked simulation of glucose-6-phosphate isomerase (GPI) primed CD4+ T cells. Mice were sacrificed on day 7 after immunisation. CD4+ T cells were purified from spleen cells of GPI-immunised DBA/1 mice. GPI-primed CD4+ T cells and antigen presenting cells (APCs) were co-cultured with 10 μM of synthetic peptide for 24 hours. The supernatants were assayed for interferon (IFN) γ and interleukin (IL) 17 by ELISA. Data are averages ± standard deviation of three culture wells. Representative data of three independent experiments.