Figure 2.
GPI325–339 is a major epitope. Mice were sacrificed on day 7 after immunisation. CD4+ T cells were purified from splenocytes of glucose-6-phosphate isomerase (GPI) immunised DBA/1 mice. GPI-primed CD4+ T cells and antigen presenting cells (APCs) were co-cultured with 10 μM of synthetic peptide hGPI325–339, hGPI334–348, hGPI542–556 or hGPI544–558 for 24 hours. The purity of each peptide was 90%. The supernatants were assayed for interferon (IFN) γ and interleukin (IL) 17 by ELISA. Data are averages ± standard deviation of five culture-wells. **p < 0.01 (Mann-Whitney's U test). Representative data of three independent experiments.