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. 2008 Nov 25;10(6):R137. doi: 10.1186/ar2560

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Copyright © 2008 Nguyen et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of anti-nuclear autoantibodies in sera of C57BL/6.NOD-Aec1RAec2R(n) mice. Serum samples obtained from C57BL/6.NOD-Aec1Aec2R(n) mice were diluted 1:40 and incubated with HEp-2 fixed substrate slides for 30 minutes at 25°C in a humidified chamber. The slides were then developed with Alexa 594-conjugated goat anti-mouse IgG and viewed by fluorescence microscopy at × 100 magnification. Examples of speckled/homogenous staining of the nucleus (left panel), cytoplasmic/nuclear membrane staining (left center panel), speckled/cytoplasmic staining (right center panel), and cytoplasmic staining (right panel) were observed. The numbers of individual sera tested and the percentages of positive sera from a sampling of recombinant inbred (RI) lines exhibiting each of the patterns are listed. ^§Number of mice showing positive staining pattern over total. *Percentage of mice showing positive staining pattern. Aec, autoimmune exocrinopathy.