Figure 2.
Polydactylous HDAC9/HDRP null mice exhibit increased SHH signaling. The indicated tissue samples were obtained from polydactylous HDAC9/HDRP null mice (-/-) and wild-type littermates (+/+) at either 4 days of age (N = neonatal) or 4 weeks of age (A = adolescent), RNA was extracted from whole organs and normalized between knockout and wild-type samples, cDNA was then made, and PCR performed using gene-specific primers.