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. Author manuscript; available in PMC: 2009 Mar 16.
Published in final edited form as: Reproduction. 2008 Apr 4;136(1):53–63. doi: 10.1530/REP-07-0514

Figure 3.

Figure 3

Granulosa cell levels of PGF2α synthesis enzyme mRNA and protein. Granulosa cells obtained from monkeys experiencing controlled ovarian stimulation before (0 hour) and 12, 24, and 36 hours after hCG were assessed for AKR1C3 mRNA (A) and protein (B) levels by real time RT-PCR and western blotting, respectively. Panel C. Granulosa cell level of mRNA for AKR1C1/AKR1C2 was determined by real time RT-PCR. Panel D. Representative gel shows cDNA amplified by real time RT-PCR representing detection of mRNA for β-actin (lane 1), AKR1C1/AKR1C2 (lane 2), and AKR1C3 (lane 3) in monkey granulosa cells; negative control is also shown (RT omitted, lane 4). Panel E. A representative western blot shows detection of AKR1C3 at 36 kD in lysates of monkey granulosa cells obtained 0, 12, 24, and 36 hours after hCG; detection of tubulin in each sample is also shown. All enzyme mRNA levels were normalized to the level of β-actin mRNA in the same sample. Western blotting data were normalized to tubulin content of each sample. Within each panel, groups with no common superscripts are different by ANOVA and Newman Keuls test, p<0.05. Data are presented as mean±SEM, n=4-5/group.