Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Placenta. 2008 Dec 19;30(2):156–162. doi: 10.1016/j.placenta.2008.11.017

Figure 4. Oral infection induced inflammation and TLR4 expression in murine placentas.

Figure 4

Histological and immunofluorescence analysis of murine placentas. 4A: Representative image depicting extensive junctional zone (JZ) along with an increased inflammatory cell infiltrate (arrowheads) in placentas from infected mice. Decidua (D); Bar=50μm. 4B–4C: immunofluorescence of a placenta from the C. rectus and C. rectus/P. gingivalis infected groups respectively. Images represent 3 merged channels (Rhodamine, FITC and DIC) depicting trophoblasts expressing TLR4 from both groups. 4D–E: images depict the expression of Cytokeratin 7 (FITC-green) and TLR4 (Rhodamine-red) in labyrinth trophoblasts from a different C. rectus-infected section; 4F: image illustrates co-localization of Cytokeratin 7 and TLR4 suggesting enhanced expression of TLR4 by labyrinth trophoblasts. 4G-I: images correspond to fluorescence negative controls (no primary antibodies used) for Cytokeratin 7 and TLR4 respectively. Rhod=Rhodamine (red); FITC= Fluorescein isothiocyanat (green); White bars=20μm.