FIGURE 1.
BMP-4 induces modest expression of Sca-1 and HOXB4. (a) Flow cytometric analysis of CD45, H-2Kb, and Sca-1 expression on untreated or BMP-4-treated 129 SvJ R1 ES cells. BMP-4 modestly up-regulated Sca-1 expression. (b) Reverse-transcriptase polymerase chain reaction of the ES cell-specific gene Nanog and the hematopoietic-specific genes BMP-4, BMPR, and HOXB4 in untreated or BMP-4-treated 129SvJ R1 ES cells. BMP-4 treatment induced HOXB4 expression in ES cells.