Figure 3. Compartmentalization of neutrophils following kidney ischemia-reperfusion injury.
We harvested kidneys and lungs 5 min after injection of anti-GR-1 antibody or isotype control in sham or 24 h renal ischemia-reperfusion injury (IRI) mice. Marginated neutrophil content was identified as 7/4+GR-1+ and interstitial neutrophil content was identified as 7/4+GR-1−. Graphs show representative dot plots after 24 h of isotype control, sham, or IRI.