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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Kidney Int. 2009 Jan 7;75(7):689–698. doi: 10.1038/ki.2008.648

Figure 4. Histological and immunofluorescence localization of neutrophils to interstitial and marginated compartments in the kidney and lung.

Figure 4

Histological staining of kidney outer medulla of mice subjected to 24 h renal ischemia-reperfusion injury (IRI) showing peritubular capillary neutrophils (a), interstitial neutrophils (b and c: arrows), and intraluminal neutrophils (b: arrowhead). Immunofluorescence staining of kidney outer medulla (d and e) and lung (f) using antibodies to 7/4 (green; for neutrophils) and CD31 (red; for vascular endothelium). Nuclei are depicted by DAPI labeling (blue). Neutrophils in both sides of the vascular endothelial wall, and circle shows a neutrophil transmigrating through the vascular wall of the kidney (d: arrowhead). Z-stack image (7.0 mm) of 12 optical slices of the kidney at 0.6 mm intervals (e). Kidney interstitial neutrophils (arrows) are shown in inset (e). Lung tissue shows all neutrophils in the vasculature (f).