Figure 5. Differential analysis of intracellular cytokine content between marginated and interstitial neutrophils.
Twenty-four hours following renal ischemia-reperfusion injury (IRI), mice were injected with APC-conjugated GR-1 anti-mouse Ly-6G antibody through tail vein. Five minutes later, mice were anesthetized and perfused, and kidneys were excised and processed for FACS analysis for intracellular cytokine content. Values are mean ± s.e.m.; n = 5 all groups. *P<0.05, **P<0.005 compared with interstitial neutrophils. Open bars, interstitial neutrophils; filled bars, marginated neutrophils.