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. Author manuscript; available in PMC: 2009 Sep 12.
Published in final edited form as: Oncogene. 2009 Jan 19;28(10):1348–1356. doi: 10.1038/onc.2008.493

Figure 4. Localization of TRIP and Syk in epithelial cells.

Figure 4

(a) Endogenous or transiently expressed TRIP in MCF7(BD) cells was detected by immunofluorescence using an anti-TRIP antibody. Nucleolin was stained with a monoclonal anti-nucleolin antibody. The nuclei were stained with DAPI. (b) MCF7(BD) cells transfected with 50 nM of TRIP siRNA or a control siRNA (Ctrl) were analyzed by immunofluorescence using anti-TRIP antibodies or by treatment with DAPI. (c) MCF7(BD) cells expressing Syk-EGFP and TRIP were treated with TNF-α (100 ng/ml) for 5 min (+TNF) or left untreated (−TNF). TRIP was detected by immunofluorescence with the anti-TRIP antibody. An example of the colocalization of TRIP and Syk is indicated. E, MCF7(BD) cells expressing Syk-EGFP and Myc-TRIP were subjected to TNF-treatment. Myc-TRIP was immunostained with an anti-Myc antibody. An example of the colocalization of Syk-EGFP and Myc-TRIP is indicated. Bars represent 10 µm.