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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Exp Eye Res. 2008 Nov 13;88(3):467–478. doi: 10.1016/j.exer.2008.10.023

Fig. 3.

Fig. 3

Dose dependence of CCH-stimulated release of vIL-10 and β-hexosaminidase from LGAC. Rabbit LGACs were seeded onto Matrigel-coated 24-well plates at 1 × 106 cells/well. On day 2 of culture, reconstituted acini were transduced with AdvIL-10 at a MOI of 5 for 24 h. Control and transduced acini were then incubated in fresh medium for 1 h to allow cell equilibration. After treatment without or with 1, 10, 100 and 1000 μM CCH for 30 min, aliquots of the medium were collected and processed for measurement of vIL-10 (A) and β-hexosaminidase (B) release using ELISA and biochemical assays, respectively. vIL-10 and β-hexosaminidase release are plotted as % of total, with total defined as the maximal release elicited by 100 μM CCH at 30 min in acini transduced with AdvIL-10. n = 4. Error bars represent s.e.m.