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. 2009 Mar 3;9:74. doi: 10.1186/1471-2407-9-74

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Copyright ©2009 Sarrió et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of E- or P-cadherin does not completely reverse the mesenchymal phenotype of 231 cells. First row: Double immunofluorescence of E- or P-cadherin (Alexa-594, red) and actin cytoskeleton (Phalloidin-Alexa-488, green). Second row: Focal adhesions (arrows) co-stained with Phalloidin (green) and anti-paxillin (red). Note that no evident changes in the number or organization of focal adhesions are seen among the different conditions. Third and fourth rows: expression of the mesenchymal marker vimentin and the epithelial marker cytokeratin.