Figure 5. Transplanted Kras^G12D Flk2⁻ LSK Cells Induce MPD.

Mice were analyzed 3 mo after transplantation with either Kras^G12D or WT Flk2⁻ LSK cells for evidence of MPD (n = 3 WT, n = 6 Kras^G12D in a single experiment).

(A) Numbers of circulating leukocytes (WBC).

(B) Spleen weights.

(C) Percentages of myeloid cells (Mac1+ and/or Gr1+) in the spleen.

(D) Percentages of erythroid precursors (nucleated and TER119+) in the spleen.

(E) A representative splenic section demonstrating infiltration by mature myeloid cells (ring-shaped nuclei) and erythroblasts (small dense nuclei).