Skip to main content
. 2009 Mar 16;106(14):5813–5818. doi: 10.1073/pnas.0810550106

Fig. 4.

Fig. 4.

Regulation of gene expression and identification of growth target genes in 375KO islets. (A) Quantification of percentage of Ki-67-positive nuclei within insulin-positive cells of 375KO (black bars) and wild-type (gray bars) male mice. (B) Analysis of gene expression of putative miR-375 targets by real-time PCR in mutant and wild-type pancreatic islets. n = 5 animals per genotype. (C) Western blot analysis of protein lysates from pancreatic islets isolated from 375KO and wild-type (WT) male mice (100 islets per lane). Quantitative measurements made from densitometry are expressed as a ratio of mean values of 375KO to wild-type mice. (D) Increase in intracellular concentration of miR-375 decreases luciferase activity in HEK293 cells transfected with reporter constructs containing either full-length or partial 3′UTR sequence of putative miR-375 target genes (n = 6). Values relative to luciferase activity from cells transfected with a scrambled control are shown. Data are presented as means ± SE. *, P < 0.05; **, P < 0.01; ***, P < 0.001.