FIG. 3.
Coculture of OT2 TCR-transduced BM cells with various OP9 cells (OP9-MIG, OP9-IAb, OP9-DL1, and OP9-DL1-IAb). Results shown is one representative set of data from three independent experiments. (A) The expansion rate of transduced BM cells following coculture on OP9 cells. Total cellularity from OP9-MIG (▪), OP9-IAb (•), OP9-DL1 (▴), or OP9-DL1-IAb (▾) coculture was measured at the indicated time points. (B) Phenotypic analysis of Sca-1 and c-Kit double-positive transduced BM cells following coculture on OP9 cells. Percentages of Sca-1+c-Kit+ cells from various OP9 cells [the labeling was the same as (A)] were determined by flow cytometry analysis during the course of coculture at the indicated time points. (C) Phenotypic analysis of committed T-cell population (Thy1+B220+) from transduced BM cells following coculture on OP9 cells. Percentages of Thy1+B220+ cells from various OP9 cells [the labeling was the same as (A)] were determined by flow cytometry analysis during the course of coculture at the indicated time points. (D) The dot plots show the flow cytometry analysis of surface coexpression of CD3 and OT2 TCR alpha chain from transduced BM cells following coculture on either OP9-DL1 or OP9-DL1-IAb at the indicated time points.