FIG. 4.
Development of CD4 T cells from TCR-transduced BM cells cocultured with either OP9-DL1 or OP9-DL1-IAb cells. Results shown is one representative set of data from three independent experiments. (A) Phenotypic analysis of double-negative development of transduced BM cells following coculture with OP9 cells at indicated time points by surface costaining of CD25 and CD44 expressions. (B) Phenotypic analysis of CD4 and CD8 expressions on transduced BM cells cocultured with OP9 cells at indicated time points by surface costaining of CD4 and CD8. (C) The total numbers of SP CD4 T cells generated from OP9 cocultures. The two million BM cells were transduced with OT2 vector and then the OP9 cocultures were initiated.