Fig. 3.

Effects of Koenig's polyanion on DNP- and ADP-stimulated respiration in digitonin-permeabilized hepatocytes. Respiration of hepatocytes (2 × 106 cells/ml) was measured in ICB supplemented with 5 mM succinate. (A) DNP-stimulated respiration was measured after sequential addition of 8 μM digitonin (Dig 8), 25 μg/ml KPA (25 KPA), and 80 μM digitonin (Dig 80). (B) ADP (500 μM)-stimulated respiration was measured after sequential addition of 8 μM digitonin (Dig 8), 25 μg/ml KPA (25 KPA) and 80 μM digitonin (Dig 80). Oligomycin and ATP were omitted from ICB for respiration experiments. Data are means ± SEM of at least six experiments. *p < 0.05 compared to Dig 8.