Fig. 4.

Restoration of ethanol-inhibited respiration by high digitonin. (A) Respiration of untreated hepatocytes (2 × 106 cells/ml) was measured in ICB (without oligomycin and ATP), supplemented with 5 mM succinate and 500 μM ADP after no addition (None) and after sequential addition of 8 μM and 80 μM digitonin (Dig 8 and Dig 80, respectively). (B) Respiration was measured as in A in hepatocytes exposed to 50 mM ethanol for 20 min. Data are means ± SEM of at least six experiments. *p < 0.05 compared to None; **p < 0.05 compared to Dig 8.