Skip to main content
. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Nov 11;481(2):226–233. doi: 10.1016/j.abb.2008.10.036

Fig. 5.

Fig. 5

Ethanol exposure limits accessibility to mitochondrial AK. Untreated and ethanol-treated hepatocytes (2 × 106 cells/ml) were incubated with digitonin (8 μM) and centrifuged. AK activity was measured in the cytosol (A) and pellet (B), as described in Materials and methods. In (B) the pellet from low-digitonin treated, ethanol-treated hepatocytes was treated additionally with high digitonin (80 μM). Data are means ± SEM of at least six experiments. *p < 0.05 compared to None. **p < 0.05 compared to ethanol.