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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Nov 11;481(2):226–233. doi: 10.1016/j.abb.2008.10.036

Fig. 6.

Fig. 6

Mechanical permeabilization and RhoDex entrapment in cultured hepatocytes. Cultured hepatocytes were loaded with green-fluorescing MTG and incubated in ICB (A) RhoDex (400 μM) was then added followed by mechanical perturbation using a micropipette. Afterwards, RhoDex penetrated the cytoplasm and nuclei (B) After 120 s, the medium was replaced with ICB containing both RhoDex and DIDS (30 μM), the latter a VDAC inhibitor to entrap RhoDex in the mitochondrial intermembrane space. After another 60 s, the hepatocytes were washed with ICB containing only DIDS to remove unbound RhoDex. After another 300 s, images of MTG and RhoDex fluorescence were collected to identify RhoDex remaining within mitochondria (C) Higher magnification (lower panels) shows retention of red-fluorescencing RhoDex (E) in MTG-labeled mitochondria (D) as evident in the overlay (F) White arrows identify strongly RhoDex-labeled structures that do not co-label with MTG.