Table 2.
DNA sequence analysis of descendants of a gap-lesion plasmid carrying a site-specific TT CPD after repair in polη-proficient and polη-deficient human cells
| DNA sequence opposite TT CPD |
Primary fibroblasts | SV40-transformed fibroblasts |
Burkitt's lymphoma | |||
|---|---|---|---|---|---|---|
| POLH+/+ | POLH−/− | POLH+/+ | POLH−/− | POLH+/+ | POLH−/− | |
| Number of isolates (%) | ||||||
| C-AA-C | 78 (99) | 54 (78) | 84 (94) | 73 (81) | 64 (89) | 44 (56) |
| A-AA-C | - | 2 (3) | - | 1 (1) | 1 (1.5) | - |
| G-AA-C | - | 1 (1) | - | 1 (1) | - | - |
| C-CA-C | - | - | - | 1 (1) | - | - |
| C-GA-C | - | 3 (4) | - | 5 (6) | - | 6 (8) |
| C-TA-C | - | 2 (3) | - | 5 (6) | - | 7 (9) |
| C-AA-A | - | 2 (3) | - | 1 (1) | - | 1 (1) |
| Δ-AA-C | - | - | 1 (1) | - | - | - |
| C-AT-A | - | - | - | 1 (1) | - | - |
| C-TA-A | - | - | - | - | 1 (1.5) | - |
| Non-TLS events | 1 (1) | 5 (7) | 4 (4) | 2 (2) | 6 (8) | 20 (26) |
| Total | 79 (100) | 69 (100) | 89 (100) | 90 (100) | 72 (100) | 78 (100) |
Plasmids were extracted from kanR colonies obtained in the experiments described in Table 1, and subjected to DNA sequence analysis. The sequence opposite the site of the TT CPD is shown in the 5' to 3' direction. Accurate TLS is represented by the sequence 5'-CAAC-3'. The underlined nucleotides are those located opposite the original TT CPD. Mutations are presented by bold type. Δ represents a single-nucleotide deletion. Non-TLS events include big deletions and insertion of sequences from the control plasmid.