Table 6.
Categories of molecular events occurring during TLS across a TT CPD in polη-proficient and polη-deficient human cells
| Cell type: | Primary fibroblasts | SV40-transformed fibroblasts | Burkitt's lymphoma cells | |||
|---|---|---|---|---|---|---|
| POLH+/+ | POLH−/− | POLH+/+ | POLH−/− | POLH+/+ | POLH−/− | |
| Category of molecular event | Number of isolates (%) | |||||
| Error-free TLS | 59 (46) | 40 (40) | 43 (47) | 22 (34) | 86 (68) | 54 (55) |
| Error prone TLS | 57 (45) | 49 (50) | 45 (50) | 40 (63) | 34 (27) | 37 (37) |
| Targeted mutations | ||||||
| Transitions | ||||||
| At 3' T | 5 | 5 | 2 | 2 | 2 | 1 |
| At 5' T | 3 | 2 | 3 | 1 | ||
| Transversions | ||||||
| At 3' T | 3 | 3 | 2 | 4 | 3 | |
| At 5' T | 5 | 1 | 6 | 3 | 2 | 1 |
| Small deletions | 1 | 4 | 2 | 6 | 12 | |
| Semi-targeted mutations | ||||||
| 3' to the lesion | 1 | 1 | 8 | 1 | 2 | |
| 5' to the lesion | 25 | 33 | 22 | 18 | 11 | 16 |
| Tandem double | 4 | 4 | 2 | 2 | 1 | |
| Non-tandem double | 10 | 2 | 1 | 6 | 6 | 2 |
| Non-TLS events (insertions or deletions) | 12 (9) | 10 (10) | 3 (3) | 2 (3) | 6 (5) | 8 (8) |
| Total | 128 (100) | 99 (100) | 91 (100) | 64 (100) | 126 (100) | 99 (100) |
Based on the data presented in Table 5.