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. Author manuscript; available in PMC: 2009 Mar 17.
Published in final edited form as: Carcinogenesis. 2007 Jul 25;28(12):2605–2613. doi: 10.1093/carcin/bgm163

Fig. 6. Suppression of MnSOD mRNA by p50.

Fig. 6

JB6 cells were co-transfected with either p50 expression vector (pcDNA3.1/p50) or empty vector (pcDNA3.1) individually or in combination with Sp1 expression vector (pcDNA3.1/Sp1) along with MnSOD reporter gene. Twenty-four hours after co-transfection, cells were treated with TPA (100 nM) for 12 h. Cells were then collected and RNA was isolated and purified. (A) Over-expressed p50 and Sp1 are shown by western blotting of cell homogenates. (B) RT–PCR of RNA isolated from control and TPA-treated cells was carried out using primers specific to mouse MnSOD gene and β-actin gene used as loading control. A representative of duplicate experiments with identical results is shown.