. Author manuscript; available in PMC: 2009 Dec 1.

Published in final edited form as: Bioconjug Chem. 2008 Dec;19(12):2487–2491. doi: 10.1021/bc800417b

Table 1.

Optical properties of pentamethine carbocyanine dyes

	λ_abs (nm)^a	λ_em (nm)	ε (M^-1cm^-1)^b	Φ^c
CyAM-5 acid	642	656	215000	0.17
CyAM-5 azide	641	658	222000	0.20
CyAM-5 alkyne	641	657	240000	0.20

Spectra were obtained in phosphate buffered solution, pH 7.0. Details are listed in experimental procedures.

Extinction coefficients at 640 nm are averaged over at least three sets of equivalent experiments. Details are listed in experimental procedures.

Emission spectra were excited at 620 nm. Details are listed in experimental procedures.