Fig. 8.

Pair-feeding studies in HFD fed WT and FASKO mice. A–E: Effect of pair-feeding WT mice to ad libitum fed FASKO animals on body weight gain (A), percent change in body weight compared with corresponding ad libitum fed controls (B), adiposity (C), percentage of lean mass (D), and oxygen consumption (by indirect calorimetry integrated over a 24 h period) (E). Results represent mean ± SEM of 7–10 animals per group. * P < 0.05 compared with WT control (Ctrl). F: Immunoblotting for ⁴⁷³Ser-phosphorylated and total Akt in the liver after pair-feeding in response to an intraportal bolus injection of 0.1 U/kg regular insulin (top panel), and corresponding densitometric quantification (bottom panel). Data represent mean ± SEM of four animals per group. * P < 0.05 vs. WT-Ctrl. ^# P < 0.05 vs. WT-pair-fed. G: Serum TNFα concentration at the indicated time points in control and pair-fed WT and FASKO mice in response to 7 mg/kg lipopolysaccharide injection. Results are mean ± SEM of 5–6 animals per group. * P < 0.05 vs. WT-Ctrl. ^# P < 0.05 vs. WT-PF. H: An integrative model for the regulatory role of brain FAS in energy homeostasis. Hypothalamic FAS deficiency impacts multiple organ systems culminating in resistance to diet-induced obesity (DIO) and increased insulin sensitivity.