Fig. 5.

TRPV1 can be activated by 3(R)-hydroxy-anandamide in whole-cell patch clamp recordings. A: Representative example of a whole cell patch clamp recording of HEK-293 cells overexpressing the human recombinant TRPV1 in response to 3(R)-hydroxy-anandamide (n = 8), here denoted as “3-HETE-anandamide.” Data are gathered from voltage-ramps and depict the current at +80 mV and −80 mV. The zero current level is indicated by the dashed line. Lowercase ‘a’ refers to traces obtained in the absence of capsazepine (CPZ); lowercase ‘b’ refers to traces obtained in the presences of CPZ. B: 500 ms voltage ramp from −80 mV to +80 mV applied at the time points indicated as in A. 3(R)-hydroxy-anandamide and the TRPV1 antagonist, capsazepine were applied at concentrations of 20 μM and 10 μM, respectively.