TABLE 1.

PE N-acylation and PLA_1/2 activities in the homogenates of COS-7 cells overexpressing recombinant human, mouse, or rat H-Rev107

	PE N-acylation	PLA1/2
	nmol/min/mg
Mock	0.040 ± 0.004	0.28 ± 0.028
Human H-Rev107	0.115 ± 0.015	13.8 ± 0.38
Mouse H-Rev107	0.133 ± 0.013	12.8 ± 0.42
Rat H-Rev107	0.109 ± 0.021	12.9 ± 0.54
Rat iNAT	0.195 ± 0.014	1.2 ± 0.14

PE, phosphatidylethanolamine; PLA_1/2, phospholipase A_1/2; iNAT, Ca²⁺-independent N-acyltransferase. The homogenates (10 μg protein) of COS-7 cells expressing the indicated proteins were assayed for PE N-acylation activity and PLA_1/2 activity as described under Experimental Procedures. Mean values ± SD are shown (n = 3).