TABLE 1.

Effects of fat and vitamin A on body weights, tissue weights, and serum vitamin A in rats fed two levels of fat and vitamin A^a

	Fat ± Vitamin Ab				Factors (P)c
Variable	NF−	NF+	HF−	HF+	F	VA	F × VA
Final body weight (g)	509.8 ± 64.4	536.1 ± 36.9	577.1 ± 33.1	593.7 ± 55.2	<0.0001	0.14	0.82
Liver weight (g)	19.3 ± 4.2	20.5 ± 2.8	22.5 ± 3.0	21.2 ± 2.9	0.047	0.97	0.19
Perirenal adipose (g)	14.3 ± 5.2	16.8 ± 3.4	21.8 ± 6.1	23.7 ± 6.9	<0.0001	0.17	0.83
Epididymal adipose (g)	9.3 ± 2.1	11.9 ± 1.9	15.8 ± 3.0	15.7 ± 4.1	<0.0001	0.15	0.12
Serum R (μmol/L)	0.50 ± 0.12	1.41 ± 0.31	0.47 ± 0.12	1.47 ± 0.34	0.86	<0.0001	0.58
Serum CRP (mg/L)d	150.3 ± 24.8	114.5 ± 20.6	143.3 ± 57.2	126.8 ± 31.6	0.86	0.094	0.52
Liver fat (mg/g)	33.3 ± 14.6	34.1 ± 11.0	44.6 ± 9.5	44.7 ± 10.5	0.002	0.88	0.93
MRDR ratioe	1.23 ± 0.37	0.16 ± 0.05	1.58 ± 0.37	0.16 ± 0.08	0.21	<0.0001	0.21

^aValues (n = 12 per treatment group) represent least squares means ± SD, with significant differences (P < 0.05) by variable.

^bTreatment groups follow: NF- 6% dietary fat, 17 nmol retinyl acetate daily; NF+ 6% dietary fat, 70 nmol retinyl acetate daily; HF- 21% dietary fat, 17 nmol retinyl acetate daily; HF+ 21% dietary fat, 70 nmol retinyl acetate daily.

^cFactors include the main effects of dietary fat (F) and vitamin A (VA) and the interaction between fat and vitamin A (F × VA).

^dSerum CRP is n = 6 per treatment group.

^eThe modified relative dose response (MRDR) ratio is the molar concentration of 3,4-didehydroretinol to retinol (R) in the serum 4 h after dosing with 3,4-didehydroretinyl acetate (n = 4 per treatment group).