TABLE 2.
Calibration equations, correlation coefficients, linear ranges, and limits of detection for n-3 PUFAs
| n-3 PUFA | Calibration Equationa | Correlation Coefficient | Linear Range | Limit of Detectionb |
|---|---|---|---|---|
| r | fg | |||
| α-LNA | y = 0.4105x + 0.0001 | 0.9973 | 4 pg−8 ng | 8.0 |
| EPA | y = 0.7084x − 0.0027 | 0.9987 | 5 pg−10 ng | 10.0 |
| DPA | y = 0.5345x − 0.0021 | 0.9935 | 5 pg−11 ng | 11.0 |
| DHA | y = 0.9395x − 0.0032 | 0.9936 | 5 pg−10 ng | 10.0 |
a
Calibration equations were determined by using standard unesterified n-3 PUFAs and internal standards, where x represents the peak area ratio of the unlabeled n-3 PUFA to the internal standard, and y represents the concentration (mol) ratio of the analyte to the internal standard, respectively.
b
Limit of detection was determined when the ratio of signal to noise was 10.