Fig. 3.

Isolation of rafts with sucrose and Optiprep™ gradients. Top panels show flotillin 1 (Flot1) and bottom panels are transferrin receptor (TfR). A: Raft isolation using a detergent-free buffer along a sucrose gradient. Flot1 is maximally present in fractions 1–5. TfR is absent from fractions 1–3. B: Raft isolation using a detergent-free buffer along an Optiprep™ gradient. Flot1 is maximally present in fractions 2–5; TfR is absent from fractions 1 and 2. Blots are representative of three individual experiments. Black bold rectangles designate raft fractions.