Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2009 Mar 17.

Published in final edited form as: Biochemistry. 2005 Dec 13;44(49):16140–16148. doi: 10.1021/bi051045l

Fig. 2 — Self –assembly of DMP1 as tracked by AFM imaging in tapping mode: A: Topographic image of the monomeric state of recombinant DMP1. A': Line analysis of A (particle size : ∼ 28.2 ± 1.9 nm; n=15). Images of oligomerized dentin in the presence of 2.5 mM Ca²⁺ after overnight incubation. B: Topography (particle size varied from 66.63 to 113.6 nm); C: Corresponding phase image. C': Line analysis from B. .D: Topographic image of the monomeric state of the 57 kDa COOH terminal region of native DMP1; (particle size : ∼ 15nm) (E): Topographic image of oligomerization of the 57 kDa COOH-terminal region of native DMP1 in the presence of 2.5 mM Ca²⁺ after overnight incubation (particle size : ∼38 nm). All images were collected at a resolution of 512×512. Scale bars are 75nm.

Fig. 2 — Self –assembly of DMP1 as tracked by AFM imaging in tapping mode: A: Topographic image of the monomeric state of recombinant DMP1. A': Line analysis of A (particle size : ∼ 28.2 ± 1.9 nm; n=15). Images of oligomerized dentin in the presence of 2.5 mM Ca²⁺ after overnight incubation. B: Topography (particle size varied from 66.63 to 113.6 nm); C: Corresponding phase image. C': Line analysis from B. .D: Topographic image of the monomeric state of the 57 kDa COOH terminal region of native DMP1; (particle size : ∼ 15nm) (E): Topographic image of oligomerization of the 57 kDa COOH-terminal region of native DMP1 in the presence of 2.5 mM Ca²⁺ after overnight incubation (particle size : ∼38 nm). All images were collected at a resolution of 512×512. Scale bars are 75nm.