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. Author manuscript; available in PMC: 2010 Feb 7.
Published in final edited form as: Cell. 2009 Feb 6;136(3):447–460. doi: 10.1016/j.cell.2009.01.014

Figure 2. The F978A mutation in eIF4G HEAT-1 affects binding to eIF4A, but not to eIF4A-CTD.

Figure 2

(A, B) Overlay of SPR sensorgrams showing binding of eIF4A to immobilized WT eIF4G HEAT-1 (A) and eIF4G HEAT-1 F978A

(B). (C, D) SPR graphs showing binding of full-length eIF4A (C) and eIF4A-CTD (D) to immobilized WT eIF4G HEAT-1 (black) and HEAT-1 F978A (red). KD values marked with a star should be considered only estimates, since concentrations higher than the KD could not be reached in the titration due to limited solubility.