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. Author manuscript; available in PMC: 2010 Jan 1.

Published in final edited form as: Mol Immunol. 2008 Nov 25;46(3):492–497. doi: 10.1016/j.molimm.2008.10.011

Fig. 2 — Organization of Dok1 and Slap1 genes to indicate location of GRE and half GRE. Upper panel: exons and introns are indicated by the numbered boxes and solid lines respectively. Dashed lines indicate non-transcribed regions. Regions that were amplified and cloned for this study are delineated and designated as A and B. These regions are highly conserved in human, mouse, and rat. Region A of the SLAP1 gene (441 bp) is a basal transcription element immediately upstream of exon 1 and region B (344 bp) contains GRE. Region A of Dok1 (2235 bp) embraces part of the promoter region that contains the half GRE. Lower panel: cloned sequences of regions A and B of the SLAP1 gene. Region B is in the reverse direction of chromosomal DNA and was inserted as such in the luciferase construct. Highly conserved binding sites for the indicated transcription factors and GR (GRE) are underlined. The + sign indicates that the consensus sequence for the binding site is in the same direction as that shown whereas the – sign indicates the reverse situation.

Fig. 2 — Organization of Dok1 and Slap1 genes to indicate location of GRE and half GRE. Upper panel: exons and introns are indicated by the numbered boxes and solid lines respectively. Dashed lines indicate non-transcribed regions. Regions that were amplified and cloned for this study are delineated and designated as A and B. These regions are highly conserved in human, mouse, and rat. Region A of the SLAP1 gene (441 bp) is a basal transcription element immediately upstream of exon 1 and region B (344 bp) contains GRE. Region A of Dok1 (2235 bp) embraces part of the promoter region that contains the half GRE. Lower panel: cloned sequences of regions A and B of the SLAP1 gene. Region B is in the reverse direction of chromosomal DNA and was inserted as such in the luciferase construct. Highly conserved binding sites for the indicated transcription factors and GR (GRE) are underlined. The + sign indicates that the consensus sequence for the binding site is in the same direction as that shown whereas the – sign indicates the reverse situation.