Table III.
Results of embryo development and fluorescence in situ hybridization (chromosomes X, Y, 13, 18, 21) in vivo and in vitro (Group 3)
| 3 patients |
||||
|---|---|---|---|---|
| 57 COC |
||||
| 47 MII oocytes |
||||
| 44 successfully injected MII oocytes |
||||
|
in vitro culture |
in vivo culture |
|||
| Day 0 | ||||
| Allocated injected oocytes | 22 | 22 | ||
| Day 1 | ||||
| 2PN oocytes | 16/22 | 72.7% | ||
| Day 3* | ||||
| Q1 | 6/22 | 27.3% | 3/22 | 13.6% |
| Q2 | 4/22 | 18.2% | 11/22 | 50.0% |
| Q3 | 4/22 | 18.2% | 5/22 | 22.8% |
| Q4 | 2/22 | 9.1% | 0/22 | 0.0% |
| Not retrieved | 3/22 | 13.6% | ||
| Day 5** | ||||
| Q1 | 2/22 | 9.1% | 3/22 | 13.6% |
| Q2 | 3/22 | 13.6% | 8/22 | 36.4% |
| Q3 | 4/22 | 18.2% | 1/22 | 4.5% |
| Q4 | 7/22 | 31.8% | 7/22 | 31.8% |
| Not retrieved | 3/22 | 13.6% | ||
| Embryos for ET | 1 | 1 | ||
| Embryos frozen | 1 | 9 | ||
| FISH results*** | ||||
| Embryos biopsied | 13/22 | 59.0% | 17/22 | 77.3% |
| Normal1/embryos biopsied | 7/13 | 53.8% | 15/17 | 88.2% |
| Abnormal/embryos biopsied | 4/13 | 30.8% | 2/17 | 11.8% |
| No FISH result | 2/13 | 15.4% | 0 | 0.0% |
| Normal/allocated injected oocytes | 7/22 | 31.8% | 15/22 | 68.2% |
1For chromosomes X, Y, 13, 18, 21.
Using Fisher’s exact probability test:
*P = 0.13, comparing the distribution of embryo quality on Day 3 in in vitro and in vivo culture.
**P = 0.28, comparing the distribution of embryo quality on Day 5 (Q1 to Q4) in in vitro and in vivo culture.
***P = 0.049, comparing normal fluorescence in situ hybridization findings in in vitro and in vivo culture.